Cryotop Vitrification as Compared to Conventional Slow Freezing for Human Embryos at the Cleavage Stage: Survival and Outcomes
نویسندگان
چکیده
منابع مشابه
Inm-5: Outcomes of Human Vitrified Embryos Transfer
The techniques of cryopreservation let embryos which cannot be immediately transferred to the patient be stored for future use. The first successful pregnancy after transfer of cryopreserved human embryos was described in 1983 by Trounson et al. and the first birth after cryopreservation was later reported in 1984 by Zeilmaker et al.). The technique has rapidly been applied in most in-vitro fer...
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Embryo cryopreservation is an important aspect of assisted reproduction. Many methods have been described, but they have been poorly investigated in randomized trials, highlighting the need for a systematic review of the literature. Meticulous electronic/hand searches were performed to locate randomized trials (RCT) comparing embryo cryopreservation methods. Primary outcomes were clinical pregn...
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Sir, In the challenging paper by Wang et al. (2008), the authors have reported about an effective method of vitrification of human ovarian tissue with direct contact of cells in liquid nitrogen, named needle immersed vitrification. Childbirth after cryopreservation of ovarian tissue is now a reality (Donnez et al., 2004; Demeestere et al., 2007; Meirow et al., 2007; Andersen et al., 2008) and t...
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Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing method with glycerol (four recipients) or dimethyl sulfoxide (three recipients) as a cryoprotectant and vitrified-warmed using the Cryotop method (five recipients) were surgically transferred into the unilateral uterine horn of recipient bitches. As a result, the morphology of embryos frozen-thawed using the slow...
متن کاملIn vitro survival of in vitro-produced bovine embryos cryopreserved by slow freezing, fast freezing and vitrification
In order to compare the survival rate of bovine embryos cryopreserved with different protocols, oocytes obtained from abattoir-derived ovaries were used for in vitro maturation (IVM), fertilization (IVF) and co-culture. Expanded blastocysts were cryopreserved by two slowfreezing [(i) 0.5 and (ii) 1.2°C/min], (iii) one quickfreezing and two vitrification [(iv) ethylene glycol + ficoll + sucrose ...
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ژورنال
عنوان ژورنال: Taiwanese Journal of Obstetrics and Gynecology
سال: 2010
ISSN: 1028-4559
DOI: 10.1016/s1028-4559(10)60060-5